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Description
α-tubulin Recombinant Rabbit mAb (SDT-R091)Product Specification Host Rabbit Antigen tubulin Synonyms Tubulin alpha 4A chain, Alpha tubulin 1 Immunogen N A Location Cytoplasm, Cytoskeleton Accession P68366 Clone Number SDT R091 Antibody Type Rabbit mAb Application WB, IHC P Reactivity Hu, Ms, Rt Purification Protein A Concentration 0. 1 mg ml Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12 months from date of receipt
Product Specification
| Host | Rabbit |
| Antigen | α-tubulin |
| Synonyms | Tubulin alpha-4A chain, Alpha-tubulin 1 |
| Immunogen | N/A |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P68366 |
| Clone Number | SDT-R091 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.1 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:4000-1:20000 | |
| IHC-P | 1:1000 |
Background
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Tubulin α- and β-subunits have molecular weights of ~ 50 kDa and are 36%–42% identical and 63% homologous. Both tubulin subunits bind guanine nucleotides. The binding to α-tubulin at the N-site is nonexchangeable, while the binding to β-tubulin at the E-site is exchangeable. Nucleotide in microtubules does not exchange with the solution, except for terminal subunits at microtubule ends.
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