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Description
Human XBP1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody for X-Box Binding Protein 1 (XBP1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of X-Box Binding Protein 1 (XBP1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human for X-Box Binding Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | X-box binding protein 1, also known as XBP1 or TREB5, is a protein encoded by the XBP1 gene. The XBP1 gene is located on chromosome 22, while a closely related pseudogene has been identified and mapped to chromosome 5. This protein is a transcription factor that regulates the expression of genes important for the normal functioning of the immune system and cellular stress responses. It is a bZIP domain-containing transcription factor. It was first identified by its ability to bind to Xbox, a conserved transcriptional element in the human leukocyte antigen (HLA) DRα promoter. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
Based on 377 reviews
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Product Reviews
★★★★★ 4
Nice scent, hopefully helps with discoloration but dissolves fast!
I’ve purchased these 4 times for my bikini line, I’m not sure if they make a difference but I like the scent so that’s why I’ve continued to purchase. The down side is that it dissolves very fast! Within 2 weeks your bar is gone..
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Reviewed in the United States on September 22, 2025
★★★★★ 3
Didn’t burn me!
UPDATE: I have been using this soap for a few months now. Usually every other day, as that's when I shower. My melisma is still in full swing. Meaning it hasn't help fade it at all. HOWEVER, I haven't had any cystic acne since using it! For that alone, I will keep using it! My skin feels extra clean using it.
Also, I tried it on my privates - since that seems to be all the rage lately. I was very dry and scratchy "down there" afterwards. To the point I needed to apply some lotion or something. (I ended up using some Medela nipple lanolin - figuring that was safe for nips and babies to eat.) If you are going to try to lighten your privates, just know you might feel scratchy, too.
Original review: Okay, I have only used this once, but I was terrified to use it after seeing other’s reactions. I did a patch test on my inner elbow, let it sit for a few minutes, washed it off and waited 24 hrs before actually using it in my face.
So far, I lathered it up and washed my face. I let it sit for about 2 mins, then washed it off. My face didn’t feel clean, so I followed up with my regular cleanser. Tomorrow, I will use my regular cleanser 1st, then use this soap.
I can’t tell if it is helping with my melisma yet, but I just wanted to say that I didn’t have my adverse reactions to it. I will update after using it more.
The soap smells good & lathers well. Don’t know about the lightening or brightening effect yet.
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Reviewed in the United States on April 6, 2021
★★★★★ 5
Effective and Gentle – Koji White Pure Kojic Acid Soap Delivers Visible Skin Brightening Results!
I’ve been using Koji White Pure Kojic Acid Skin Brightening Soap for a few weeks now, and I’m seriously impressed with the results! If you're looking for a gentle yet effective way to exfoliate, brighten, and improve the appearance of your skin, this soap is definitely worth trying.
First of all, the soap is formulated with kojic acid, a powerful ingredient known for its ability to brighten skin and reduce dark spots, hyperpigmentation, and uneven skin tone. I’ve struggled with some dark spots and blemishes on my face, and after using this soap consistently, I’ve already noticed a noticeable difference. My skin looks brighter, more even-toned, and feels much smoother.
The soap itself has a glycerin base, which makes it incredibly gentle on the skin. Despite its exfoliating properties, it doesn’t feel harsh or drying like some other exfoliating soaps I’ve tried. In fact, it leaves my skin feeling moisturized and soft, not tight or stripped. I appreciate that it doesn't have any harsh chemicals, making it suitable for both face and body.
The lather is rich and creamy, and a little goes a long way, which makes the bar last for a decent amount of time. I use it in the shower and also apply it to my face as part of my evening skincare routine. It rinses off easily without leaving any residue behind.
I also love that this soap is non-irritating, even on my sensitive skin. It doesn’t cause redness, breakouts, or any dryness, which can sometimes happen with other brightening or exfoliating products. It’s perfect for daily use, and I've felt comfortable using it both in the morning and evening.
If you're looking for a natural and affordable way to brighten your skin and fade dark spots, I highly recommend Koji White Pure Kojic Acid Skin Brightening Soap. It’s effective, gentle, and delivers visible results without being harsh on your skin. After a few weeks of use, my skin feels healthier, brighter, and more radiant. Definitely a staple in my skincare routine now!
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Reviewed in the United States on November 15, 2024
★★★★★ 1
Breaks your skin out!!! Made in THAILAND not Japan
I waited a while to write a review of this Kojic Acid soap because I wanted to give it time to...work. I ordered the soap on TWO separate occasions within a span of six months. There are two bars in the box and both times I ordered, it was delivered on time, and well packaged.
Why did I order this soap? I have Keratosis Pilaris and was hoping to find a soap that could help with exfoliation, as well as helping lighten hyperpigmented areas like between my thighs,and other scars or marks left behind from skin breakouts. Usually,I use glycolic peels and lactic acid to help with hyperpigmentation since I have more of a chocolate complexion. So I thought I would give this soap a try.
I will begin with the positives. First,this soap is nicely packaged and smells like...ORANGES! The first month,I used the soap... not everyday but a few times a week. I LOVED IT! Between my thighs is lighter now and my skin would feel exfoliated after leaving it on for about five minutes,and then rinsing it off.
THEN.....
After a few months of using it, MY SKIN STARTED TO BREAK OUT. I'm talking about first,my skin would become EXTREMELY ITCHY. Then little bumps would pop up all over my body. I never had blemishes on my chest area. NOW I DO. I wanted this soap to help me get rid of blemishes! Not to cause them. Even as I type out this review,my skin is itching terribly! I used the soap this morning. My skin is always a bit dry afterwards,but then I use lotion or oil to moisturize. At this point,I am SURE it is this soap that is causing grief.
Since I am dark skinned,I now have to repair the damage done from this soap. It's upsetting honestly. I know some people will STILL purchase this soap even though there are numerous reviews of it breaking peoples skin out.But just know,you've been warned about the adverse affects.
I'm going to go back to using glycolic acid and lactic acid to help fade blemishes and exfoliate. I will never purchase this soap again.
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Reviewed in the United States on June 17, 2018
★★★★★ 5
Best Soap
Great soap. Eliminates old person smell and lightens dark spots.
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Reviewed in the United States on April 14, 2026