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Description
Human sTNFR2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Cell culture supernatant: Centrifuge at 1000 × g for 20 minutes. Remove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with soluble tumor necrosis factor receptor 2 (sTNFR2) capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of soluble tumor necrosis factor receptor 2 (sTNFR2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Soluble tumor necrosis factor receptor 2 ELISA kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Soluble tumor necrosis factor receptor 2 (sTNFR2), also known as tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) and CD120b, is one of the two membrane receptors that bind tumor necrosis factor-α (TNFα). Like its counterpart, tumor necrosis factor receptor 1 (TNFR1), the extracellular region of TNFR2 is composed of four cysteine-rich domains that bind to TNFα. TNFR1 and TNFR2 exhibit distinct functions when bound to TNFα due to differences in their intracellular structures, such as the lack of a death domain (DD) in TNFR2. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant and other biological fluids |
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4.3 ★★★★★
Based on 370 reviews
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Product Reviews
★★★★★ 5
Great value, excellent product!!!!!
Scent: Mango & Hibiscus, Size: 12 Fl Oz (Pack of 1)
This is the best shampoo I’ve ever used.
No joke.
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I used to spend $42 per bottle of Pureology Hydrate Sheer for years. One day I forgot to bring shampoo when I travelled and considering I am allergic to most shampoos since I was a child, I went to Whole Foods Market and bought this, as I already use the excellent Babo 50 SPF sunscreen.
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That was over 4 years ago and I’ve bought this on repeat. Just bought 2 more bottles because I fear they stop making this.
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The package is a little quirky. So I put it in a reusable pretty bottle in my shower.
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My hair is ash blonde, baby fine and highlighted, about shoulder length. This shampoo is safe for daily use, it is lightly scented with a very faint mango/flower scent that is barely there after you rinse it.
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I’ve experience no build up.
Before using this, when I was using Pureology, I had to use a clarifying shampoo once a week, otherwise my hair felt like greasy straws.
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Since using this shampoo, I no longer have to do that. My hair is clean, soft, can’t understand the volume and shine after I use this.
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My $200 per haircut hair stylist kept paying me compliments, thinking I’ve upped my hair routine with masks, heat protecting spray and all the nonsense. NOPE. I just wash with this shampoo.
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Ps- sometimes I use Babo’s Baobab & Rosehip Conditioner in the light peachy pink container, sometimes I use another conditioner. Regardless, I’d pay twice as much for this shampoo.
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Exceptional.!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 18, 2026
★★★★★ 5
Color-treated hair that looks fabulous!
Scent: Mango & Hibiscus, Size: 12 Fl Oz (Pack of 1)
I received a small sample of this shampoo as part of a Whole Foods 12 Days of Beauty holiday promotion. Normally those boxes are loaded with third-rate products the store is trying to promote. Well, not this time.
I was desperate to find a shampoo that would clean my color-treated but very, very fine hair without stripping the color or weighing it down with far too much moisture - bordering on greasy.
This shampoo has really been a game changer for me (going grey or shaving my head were considerations). It gently cleans my hair (and scalp) with no down-side results, and smells divine. My hair looks healthy & fluffy (no greasiness!) all day long and even into the following day. Added bonus - genuine shine.
I must add that I have pretty short hair so I honestly don’t need much to clean me up, although I do wash it daily. The amount in the carton is smaller than a lot of brands out there but, in my situation, I feel I am getting value for my money. My expensive dye-job looks awesome every single day - so you’ve gotta weigh your choices.
It’s working well for me and it just might be a solution for you but you won’t know unless you try it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 5, 2025
★★★★★ 5
Nourishing mango & hibiscus - LOVE!!
Scent: Mango & Hibiscus, Size: 12 Fl Oz (Pack of 1)
I was looking for a clean nontoxic alternative to my usually shampoo and conditioner. I’ve alternated around between the expensive salon-grade and Sephora products for the past 10 years. I thought I’d give Babo’s new shampoo and conditioner a try because I love the body wash. I love this stuff! It makes my hair so clean and soft. For reference, my hair type is straight and fine, but I have a lot of hair. I’m brunette and haven’t colored it in over a year.
I noticed the shampoo doesn’t foam up as much as other shampoos I use, but my hair still comes out clean and I can usually go 5 days between shampooing again (I double shampoo my roots which helps).
I also noticed the conditioner is a little more on the milkier/thinner side, which makes sense for the products to have more flow to them because they are in cartons meant to pour.
I still use my usual leave-in conditioner spray and some hair oil after I wash and it all works great together.
Overall I love the shampoo + conditioner, they smell great, they cost less than my usual products and they’re clean ingredients so it’s a win win. Worth a try!
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Reviewed in the United States on March 15, 2024
★★★★★ 4
Good shampoo. Leaves hair clean!
Scent: Mango & Hibiscus, Size: 12 Fl Oz (Pack of 1)
I love the smell of that shampoo! Not too strong.
My hair feels clean and healthy.
The packaging is cool however not so practical.
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Reviewed in the United States on August 20, 2025
★★★★★ 5
Great product
Scent: Mango & Hibiscus, Size: 12 Fl Oz (Pack of 1)
Excellent product. It's very gentle on your hair, even if used in hard water. It's over priced though, and there isn't very much in the container.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 7, 2026
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