Rat NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit (Small Sample)

Rat NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit (Small Sample)The Rat NFB p65 (Nuclear Factor Kappa B p65) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat NFB p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat NFB p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate

The Rat NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.