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Ships within 48 hours · Estimated delivery Jul 9 - Jul 14
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Description
Free TGF-Beta1 (Free Transforming Growth Factor Beta 1) ELISA KitThe Free TGF Beta1 (Free Transforming Growth Factor Beta 1) ELISA Kit measures Free TGF Beta1 in Universal Mammalian (Non Avian) Serum, Plasma, Cell Culture Supernatant, or other Biological Samples In Vitro. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Free TGF 1 antibody. Add standard and properly diluted sample into relevant well
The Free TGF-Beta1 (Free Transforming Growth Factor Beta 1) ELISA Kit measures Free TGF-Beta1 in Universal Mammalian (Non-Avian) Serum, Plasma, Cell Culture Supernatant, or other Biological Samples In Vitro. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Free TGF-β1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Free TGF-β1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Free TGF-β1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
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