Mouse Beta-CTx (Beta Crosslaps) ELISA Kit

Mouse Beta-CTx (Beta Crosslaps) ELISA KitThis Mouse Beta CTx (Beta Crosslaps) ELISA Kit uses the competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Mouse Beta CTx. During the reaction, Mouse Beta CTx in the sample or standard competes with a fixed amount of Mouse Beta CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse Beta CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin

This Mouse Beta-CTx (Beta Crosslaps) ELISA Kit uses the competitive ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Beta-CTx. During the reaction, Mouse Beta-CTx in the sample or standard competes with a fixed amount of Mouse Beta-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse Beta-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse Beta-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.