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Rat LL-37 (Antibacterial Protein LL-37) ELISA Kit (Small Sample)The Rat LL 37 (Antibacterial Protein LL 37) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat LL 37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat LL 37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
The Rat LL-37 (Antibacterial Protein LL-37) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.
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4.3 ★★★★★
Based on 632 reviews
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Product Reviews
★★★★★ 5
Paleo Perfected - With the Science to Back It Up!
Format: Hardcover
After experimenting with other versions of the paleolithic-based diet, I was happy to finally come upon Paul and Shou-Ching's book, The Perfect Health Diet. It's a scholarly treatment of a complex subject, but presented in a way that's easy to follow and understand. It combines the best parts of old-time wisdom with cutting edge science to lay out a lifestyle and an approach to health that treats the body holistically.
The authors systematically lay out the scientific and evolutionary basis for their approach to health. The fact that they both suffered from serious medical conditions - which were cured by taking this new approach - lends a great deal of sincerity to the writing. In a logical, orderly fashion the book walks through the basis for the diet, then tackles each of the major energy sources (proteins, carbohydrates and fats). Once that groundwork is laid, they address the hidden toxins that are present in most of the foods that comprise the Standard American Diet (SAD - a rather appropriate acronym) and help the reader understand HOW the foods contribute to poor health.
Throughout the book, the authors use simple but effective "call out" boxes to highlight some key science behind the diet. They also sprinkle in just the right amount of reader testimonials to highlight how their lives were changed by following the Perfect Health Diet. Those examples are placed in just the right spots so the reader can see how the concept being discussed relates to real people in the real world. The inclusion of a Takeaway at the end of each chapter helps tie all the concepts together perfectly.
My only complaint is that they use the word "diet" in the title. While they use it in the academic sense (i.e., the foods that you habitually eat to provide sustenance), too many people only think of "diet" as a restriction in your food intake to promote weight loss. I prefer to think of the authors' book as a "lifestyle", rather than simply a prescription for changing your food intake. That view is reinforced by the holistic treatment of the impacts of circadian rhythm disruption on health - a fascinating chapter that probably could have as much impact on your overall health as your choice of food! I think it's very telling that only a short chapter is actually devoted to the discussion of weight loss -- because it is based upon all of the other principles that have already been outlined. The discussion in the weight loss chapter on the scientific origins of obesity is fascinating and sobering at the same time.
Overall, the Jaminets have done a fabulous job of scouring the scientific literature and tying together some popular concepts (the whole Paleo movement continues to gain steam in mainstream society) with a solid scientific underpinning that will go a LONG way toward helping people understand not just WHAT to eat (or not eat) but also WHY.
I highly recommend this book to anyone who wants to understand how their body functions and how the food you eat impacts your health and longevity. It is well-written, thorough and very convincing!
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Reviewed in the United States on January 8, 2013
★★★★★ 5
Really enjoyed this book and found it helpful
Format: Kindle
The first night after starting this diet I found myself eating a juicy half pound hamburger without the bread, a sweet potato with butter, a small salad with fresh lemons squeezed on top and some sparkling water. Life could be a lot worse!!!! This book was written by 2 Harvard educated scientists. It is thoroughly researched and made a ton of sense to me. I've dieted a lot in my life. I always lost weight and always gained it back. That's a pretty frustrating way to live. The weight gain and loss roller coaster is awful. Over the last 20 years I've tried Atkins, Paleo Diet, Zone Diet, Weight Watchers, Nutrisystem, South Beach Diet, Mediterranean Diet, Blood type Diet etc etc etc....They all work and they all have flaws which ultimately caused me to stray from them for one reason or another. As an example Nutrisystem is convenient but I don't want to eat that food forever. Atkins and Paleo cut out major food groups PERMANENTLY! They are way too extreme for me. Zone Diet was too much work. Weight watchers with the points was too time consuming. South Beach Diet was a nice fad but I got tired of that also. Anyway, without dragging on too much longer I want to say that the best part of the Perfect Health Diet is its SIMPLICITY! This way of eating doesn't require much thought, planning, or effort. The authors give you a list of foods to eat without asking you to eliminate carbs, fatty foods, meat or dairy. I was taught what carbs to eat and avoid and in what portions. I learned what fatty foods to eat and how often to eat them. I learned about about protein and was taught the right amounts of protein to eat. Most importantly i never feel hungry on the Perfect Health Diet!
This diet WILL reduce your food cravings. You are fed well. You eat a lot of fatty foods and still lose weight. You can have dairy in the form of yogurt. You can have starches. You eat fruits and veggies along with lots of eggs, meat, and some fish. Basically you feel healthy because your body gets everything it NEEDS to make you feel GOOD! This diet is the only one I've tried that eliminates food cravings and hunger. I'd give this book 10 stars if I could
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Reviewed in the United States on June 8, 2015
★★★★★ 5
The Best, Readable Single-Source Book On Diet and Health
Format: Hardcover
As other reviewers have said, this is really the best "Paleo 2.0 book" available. For those who haven't yet jumped on the bandwagon, eating like our ancient Paleolithic (and Mesolithic) ancestors will help us avoid many of our chronic diseases and feel good doing it!
And yet, the Jaminets also take the approach that a straight meat and veggies diet is simply too low in carbs for a properly functioning immune system and proper thyroid level (carbs are required for basic immune functions like phagocytic attack and mucus secretion, as well as neurological and immune energy which feeds back to thyroid output). They like carb sources found in the paleolithic and mesolithic cultures such as tubers, fruit, dairy (for some) and low-in-antinutrient white rice.
Now, to the practical: on this diet my family and I have seen an increased level of energy and overall spunk (skin shine, sleep quality, etc.) compared to when we were on, in sequence, a paleo Zone type of diet (Barry Sears' Zone diet using paleo foodstuffs), and a lower carb Mark Sisson-style primal diet. Basically, this has more saturated fat than the former and more carbs than the latter. We are as lean as we were, but feeling better and without any problems, whatsoever.
For those who have chronic infections (read: "lingering health issues"), this book also explains how you can fine-tune your diet and supplementation to fight these infections, whether they are viral, fungal, or bacterial (a different approach for each). The same for athletes.
My only criticism is that they only give 1 sentence to the classes of diseases which fare better avoiding starch and, instead, getting their carbs from fruit and honey (such as Ankylosing Spondylitis, Psoriatic Spondylitis, and other within the class; as well as some other diseases). While this diet/lifestyle is very easy to combine with a GAPS approach, with carbs from simply sugars and not starch, this goes unsaid and the authors tend to prefer starches for most.
In short, this is the best book out there for a well-researched, unbiased view on diet and how it impacts our lives and health.
I actually recommend for those who really care to read their blog, and then go buy the book for the relatives who probably won't read the blog on their own.
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Reviewed in the United States on January 17, 2013
★★★★★ 3
One of the better food, diet, and health books available, but with significant drawbacks
Format: Kindle
Paul and Shou-Ching Jaminet present the most cogent argument in favor of ancestral-style eating that I have ever read. In fact, I never really understood the logic behind Paleo until I read Perfect Health Diet. I applaud their scientific rigor and willingness to challenge dietary dogma, Paleo or otherwise.
There is no question that removing all grains and legumes from one's diet is incredibly effective for people with autoimmune diseases and some other chronic, intractable health conditions. But when taking the evolutionary perspective on human diets it's also important to recognize that many cultures have thrived on grains and legumes for millennia, and humans have evolved some clever ways to extract nutrients from foods that would otherwise be toxic through the use of prolonged soaking, sprouting, and fermentation, all techniques that are very effective at reducing seed toxicity. I think that properly prepared grains and legumes can be a nutritious part of our diets, at least for those of us who do not suffer from autoimmunity. Stephen Guyenet has written excellent posts on legumes (wholehealthsource.blogspot.com/2013/11/beans-lentils-and-paleo-diet.html) and grains (wholehealthsource.blogspot.com/search/label/lectins).
I also wish that the Jaminets had delved a little bit deeper into the subject of fatty fish from a sustainability standpoint. They recommend that we eat fatty fish, but make no mention of seafood sustainability and the importance of avoiding farmed fish, especially farmed (aka "Atlantic") salmon. Farmed fish are fed soy, corn, and pellets made of smaller fatty fish like anchovies and sardines. Entire small fish fisheries off the coast of Western South America are being destroyed in order to feed our hunger for farmed salmon, while we have plenty of small fatty fish we can eat (but choose not to) here on the west coast of North America (herring, sardines, anchovies, etc.). I bet that the fat profile (3:6 ratio) of farmed salmon is not nearly as good as that of wild salmon and smaller cold water fish.
That said, I highly respect and follow their writing and will continue to look forward to their excellent work.
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Reviewed in the United States on January 28, 2014
★★★★★ 5
Understanding and Making the Best of Our Emotions
I am honored to write a review on this book, as I am heartfully thankful for how my mind was opened to a better consciousness of emotions.
It was amazing to realize that our emotions are not an innate part of us, but have been established from interactions with others, especially early in our life by learning from our parents and siblings. Learning emotions is a part of our thinking process. With this knowledge, my approach to emotions has fundamentally changed. Emotions are how we react and how we perceive truth. Emotions become a part of who we are and how others perceive us.
The book enlightened my perception and gave me a whole new way of viewing myself and how my emotions may not have matured. With this understanding, the book gives us advice on how to learn to improve (mature) our emotions. First by knowing and followed by determination to learn. The presentation of methods to make improvements with planning and accountability to oneself gives hope and is reassuring.
We can learn to mature our emotions. When we have achieved our goals of self-emotional maturity, the book further has methods to share with others (especially family) to bring us together in a positive open way.
This book has been a real eye-opening to me. I recommend the reading of this book to spread this awareness. A positive way forward with means to achieve a better life. The book is very thorough and provides documented ways to do the planning and accountability to accomplish goals. Always thinking positively.
My thanks to the Author.
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Reviewed in the United States on September 30, 2025