QuicKey Pro Human IL-6 (Interleukin 6) ELISA Kit

QuicKey Pro Human IL-6 (Interleukin 6) ELISA KitWith about 148 known citations, the QuicKey Pro Human IL 6 (Interleukin 6) ELISA kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human IL 6. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IL 6 are added to the micro ELISA plate wells. Human IL 6 in samples (or standards) combines with the coated antibody and HRP linked

With about 148 known citations, the QuicKey Pro Human IL-6 (Interleukin 6) ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IL-6 are added to the micro ELISA plate wells. Human IL-6 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IL-6.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ±2 nm. The OD value is proportional to the concentration of Human IL-6. The concentration of Human IL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.