SKU: 44317526333

Human SEMA3E (Semaphorin 3E) ELISA Kit

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Description

Human SEMA3E (Semaphorin 3E) ELISA KitThe Human (SEMA3E) Semaphorin 3E ELISA Kit measures Semaphorin 3E in samples. The plate has been pre coated with Human SEMA3E antibody. SEMA3E present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA3E Antibody is added and binds to SEMA3E in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SEMA3E antibody. After incubation unbound Streptavidin HRP is washed away during a

The Human (SEMA3E) Semaphorin 3E ELISA Kit measures Semaphorin 3E in samples. The plate has been pre-coated with Human SEMA3E antibody. SEMA3E present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA3E Antibody is added and binds to SEMA3E in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA3E antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SEMA3E. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

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SKU: 44317526333

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