Human CHD5 (Chromodomain Helicase DNA Binding Protein 5) ELISA Kit

Human CHD5 (Chromodomain Helicase DNA Binding Protein 5) ELISA KitThe Human CHD5 (Chromodomain Helicase DNA Binding Protein 5) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human CHD5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CHD5 and Avidin Horseradish Peroxidase (HRP) conjugate are added

The Human CHD5 (Chromodomain Helicase DNA Binding Protein 5) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CHD5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CHD5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CHD5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CHD5. You can calculate the concentration of Human CHD5 in the samples by comparing the OD of the samples to the standard curve.