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Description
Human TNNT2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Troponin T Type 2, Cardiac (TNNT2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Troponin T Type 2, Cardiac (TNNT2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Troponin T Type 2, Cardiac ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Cardiac troponin T (TNNT2 or cTnT) is a 35.9 kDa protein composed of 298 amino acids. Cardiac troponin T is the largest of the three troponin subunits (cTnT, troponin I (TnI), and troponin C (TnC)) found on cardiac actin thin filaments. As part of the troponin complex, it regulates muscle contraction. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.1 ★★★★★
Based on 810 reviews
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Product Reviews
★★★★★ 4
Have to use the USB Receiver on the Front of Your PC
Size: One Size
This is a great mouse as long as you plug the little USB receiver into the front of your pc and not the back. Plugged into a front port, and the thing just flew, smooth as silk. I moved the receiver to a port on the back, and it got choppy, would freeze, and was really slow.
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Reviewed in the United States on April 19, 2026
★★★★★ 5
Good Value, Easy Set up, Tesponsive.
Size: One Size
Works great. Comfortable, responsive, and easy set up.
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Reviewed in the United States on April 26, 2026
★★★★★ 5
Excellent mouse and more!
Size: One Size
The Logitech M510 wireless mouse is a very comfortable and smoothly performing mouse. It is a full size mouse that nicely fills up the palm of the hand. The weight of the mouse is "just right". The mouse body is symmetrical so that will be equally comfortable for left or right handed people.
The mouse movement and scroll wheel work perfectly smoothly with no jump or jitter. The mouse optical performance is very robust on poor surfaces. It works equally well on a mousepad, a bare table, on a pants leg, back of a book, even the palm of the hand. It tracks perfectly on almost any surface (which many other mouse brands have trouble with).
These mice use the Logitech "Unifying" USB mini receiver which can support up to six Logitech devices by itself. If you have a Logitech mouse, keyboard, trackball, etc... they can all operate from the same receiver (saves using up USB ports). Be aware that the unifying receiver ONLY works with Logitech products and that it is NOT Bluetooth or Wi-Fi. Logitech products can be "paired" with the unifying receiver in Windows and MAC (of course) and also in Linux by using the software package called "solaar" (free, open source). The mouse comes pre-paired to the receiver, so it works "out of the box". If you want to add (up to 5 more devices), you have to pair them yourself.
The battery life is awesome. Because I hate leaking batteries, I always use AA lithium cells for everything (including the mouse). My first old Logitech MS-510 mouse that I purchased over three years ago is still working fine on the FIRST batteries that I installed in it (two AA lithium). Since I use it on my desktop the mouse power switch is always left on. It must consume practically no power when not being used.
If you use the mouse with a laptop or need to move it from computer to computer, the little unifying receiver can be stored inside the mouse (in a little USB sized slot next to the batteries) to avoid losing it.
What follows is some technical information that you can skip if you don't care.
Construction: This mouse is very nicely engineered and can be completely disassembled by removing two little screws inside the battery compartment. No screws hide under the teflon slider pads, so those don't get damaged if you take the mouse apart. Every component then can be removed simply by sliding or snapping it out. Why take it apart? See next.
My personal feeling is that the left and right mouse buttons are too light (i.e. press too easily). The buttons are no lighter than other mice, I just prefer them a bit tighter. To change this, I replace the two little microswitches with Omron Series D2F-01 parts that have a 150 gf actuation force. It takes about twice the pressure to click these switches as compared to the originals. To replace them is super simple: Just unsolder the originals and solder the new ones in place. If you forget which way they go in, the orientation is printed on the PC board.
The side "scroll" buttons already use heavier pressure microswitches, so I don't change these. The "middle button" (pressing the scroll wheel) and the left/right scroll switches (rock the scroll wheel left or right) are a different type of switch that also do not need changing.
If you disassemble the mouse, be aware that the little plastic slider for the power switch will be free to fall out when the mouse PC board is removed. If you don't know this, it's possible for the slider to silently fall out, never to be seen again.
I won't tell you HOW to unsolder and resolder the button microswitches, because if you don't know how, you shouldn't be doing jt in the first place (or get an electronics-knowledgable friend to help). And, of course opening and modifying the mouse will void the warranty!
I will give you one important hint: Because the mouse was assembled using RoHS compliant lead free solder, the microswitch solder joints should first be RE-SOLDERED with leaded solder before sucking or wicking the joints clean. This is done to "alloy" the high temperature lead free solder into more manageable leaded solder to avoid burning/ruining the (single sided) PC board.
Good luck! This mouse (hacked or not) gets 5 stars and both thumbs up. It works flawlessly, it's comfortable, battery life is amazing and the price is good (even better when it's sometimes on sale). Grab one!
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Reviewed in the United States on December 27, 2020
★★★★★ 5
Excellent mouse, good size, enough buttons
Size: One Size
Easy to setup. Fit hand well. Has enough buttons to do everything I want. Good price.
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Reviewed in the United States on May 14, 2026
★★★★★ 5
10/10 work mouse
Size: One Size
Lasts forever. Been my work mouse for years. Batteries last forever in it. Cheap enough to keep a spare in my laptop bag for when I need to travel for work. Not a gaming mouse, but a serviceable work mouse. But not tiny like a "travel mouse" so it isn't uncomfortable to use. A man can use one without his hand cramping up.
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Reviewed in the United States on May 12, 2026